常見例句Objective To establish a in situ PCR (ISPCR) method for detecting of Borna disease virus (BDV) RNA in the cells. 目的建立檢測博爾納病病毒(BDV)RNA的原位PCR方法。Conclusions ISPCR could be applicable in detecting the DNA of T.gondii in paraffin embedded sections as a new method. 直接法原位PCR為檢測病理標本中的弓形蟲DNA提供了一種新的可行方法。Results DNA of T.gondii were detected.Form All the 18 secxions by using ISPCR,while(11/18)were positive by immunohistochemical detection. 18例直接原位PCR法檢測標本中均得到陽性信號,病原體檢出率為100%25,優(yōu)于免疫組化法(11/18)。Methods Ordinary PCR, Southern blot and direct ISPCR were applied and compared in detecting Hib in 100 paraffin-embedded lung tissues of autopsy children died of CAP. 方法用普通PCR、Southern雜交和直接ISPCR三種方法檢測100例死于肺炎的兒童尸檢石蠟包埋肺組織標本中的Hib,分析其致病、致死及在肺組織中的分布情況,并對這些方法對比分析。in situ PCR(ISPCR) 原位聚合酶鏈式反應 返回 ISPCR