常見例句Phagemid with polylinker in lacZ' for production of ssDNA. 相關(guān)說明: vector permitting production of single-stranded DNA ;LacZ gene was not detected in the two control groups in all organs at 7 day. 結(jié)論經(jīng)動(dòng)脈途徑注入重組腺病毒,其體內(nèi)的表達(dá)具有器官靶向性。LacZ gene was inserted into TK gene deleted transfer vector, the TK /LacZ+ /GTPV-TY mutant was screened. 將LacZ基因表達(dá)盒插入TK基因缺失轉(zhuǎn)移載體,篩選TK基因缺失的GTPV-TY突變株。The dnaA gene with LacZ promoter was cloned into pUC19 using the Escherichia coli DH5a strain. 將帶有LacZ啟動(dòng)子的dnaA基因構(gòu)建到pUC19中,得到重組質(zhì)粒pUCA12。The damage effect of plasmid DNA and mutation induced of lacZ gene were analysesed by low power HeNe laser radiation invitro plasmid DNA. 利用低功率HeNe激光輻照離體質(zhì)粒DNA,分析了質(zhì)粒DNA的損傷效應(yīng)和lacZ基因的突變。SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively. RT-PCR和lacZ報(bào)告基因的分析結(jié)果顯示;在表皮葡萄球菌ATCC35984中;SarA對(duì)atlE(自溶酶基因)表達(dá)起負(fù)調(diào)控作用;而對(duì)lipA(脂肪酶基因)和zinC(膜相關(guān)鋅金屬蛋白酶基因)的表達(dá)則有正調(diào)控作用. 返回 Lacz