常見(jiàn)例句Objective To investigate the value of neutral red uptake assay(NRU) in detecting the vacuolating activity of VacA. 目的 分別采用細(xì)胞計(jì)數(shù)法及中性紅攝入法 (NRU)檢測(cè)幽門螺桿菌 (Hp)空泡毒素活性 ,探討NRU檢測(cè)空泡毒素活性的應(yīng)用價(jià)值。Methods Administered dose of PERC to NHEK were determined according to neutral red uptake(NRU)assay with free serum culture in vitro. [方法]用0.;25%25的胰蛋白酶經(jīng)冷溫兩步消化皮膚;分離得到NHEK;進(jìn)行體外無(wú)血清培養(yǎng); 用中性紅吸附試驗(yàn)(NRU)測(cè)定PERC對(duì)NHEK的中性紅吸附減少50%25的濃度(NR50值);并據(jù)此確定PERC細(xì)胞毒性試驗(yàn)的染毒劑量; 返回 NRU