常見例句Conclusion The constructed recombinant fowlpox virus showed good biosafety to pregnant guinea pigs and their offspring. 結(jié)論構(gòu)建的重組雞痘病毒生物安全性好,對妊娠豚鼠和子代豚鼠無安全威脅。The nucleotide sequence of BamHI genomic fragment from fowlpox virus(FPV)strain 282E 4 has been determined by ABI PRISM TM 377 DNA sequencer. 本實驗將我國FPV282E4 株基因組3 .;6kb BamHID片段利用DNA測序儀進行了序列測定。We used the same method as above-mentioned and obtained the recombinant fowlpox virus expressing antigen part of HA gene, rFPV-12LS-HAA. 將質(zhì)粒pcR2.;1一HAA用BamHI酶切;回收純化1Marek"s disease;glycoproteinB(gB);avian influenza virus;Hemagglutinin (HA);Newcastle disease;Fusion protein(F);recombinant fowlpox virus. 01馬立克氏病,糖蛋白B,禽流感,血凝素基因,雞新城疫,F基因,重組雞痘病毒Fadly A M,Witter R L,Simth E J, et al .An outbreak of lympomas in commercial broiler chickens vaccinated with a fowlpox vaccine[J].Avian Pathol, 1996,25:35-47. 何勇群;張中直;楊漢春.;北京地區(qū)雞群網(wǎng)狀內(nèi)皮增殖病感染的血清學調(diào)查研究[J]To construct the recombinant fowlpox virus expression plasmid pUTA-GAG-IL6, IL-6 and HIV-1 gag gene were inserted into the downstream of P7.5 tandem promoter and combined promoter ATI-P7.5 of fowlpox virus expression vector pUTAL respectively. 分別將IL-6和HIV-1 gag基因插入到雞痘病毒表達載體質(zhì)粒pUTAL串聯(lián)啟動子和復合啟動子(ATI-P7.;5)下游;構(gòu)建了重組雞痘病毒表達質(zhì)粒pUTA-GAG-IL6。 返回 fowlpox