常見例句Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法:采用多聚酶鏈反應(yīng)(PCR),變性梯度凝膠電泳(DGGE)和DNA測序等方法對上海地區(qū)血友病甲基因突變進行檢測。Denaturing gradient gel electrophoresis(DGGE) is an advanced molecular fingerprinting technique. 變性梯度凝膠電泳(DGGE)是近些年微生物分子生態(tài)學(xué)研究中的熱點技術(shù)之一。The results showed that soil samples collected from same area had the high identity in DGGE bands patterns. 結(jié)果表明,采自同一地區(qū)的土壤樣品,其DGGE圖譜的相似性很高;The DGGE band number fromthe subsoil samples was obviously more thanthat fromthe soil samples around the pond. 底泥樣品的DGGE條帶數(shù)明顯多于池塘周邊土壤樣品。Results shown that the PCR product of bacterial nucleic acid, which was purified with modified CTAB method, obtained stablethe most bands in DGGE. 結(jié)果表明,改良CTAB法抽提獲得細菌核酸的PCR產(chǎn)物在DGGE凝膠上獲得的條帶最多,結(jié)果穩(wěn)定。What could you conclude from PCR/DGGE analysis of a simple that yielded one band by PCR and one band by DGGE?One band by PCR and for band by DGGE? 前者說明該條帶具有固定的序列及片段大小相同,后者說明片段大小雖然相同,但序列還是存在差異。 返回 DGGE