常見例句However, the affinity of ScFv was lower than that of Fab. FV能特異性地與角蛋白結(jié)合而與其它抗原不結(jié)合,說明人源性抗角蛋白Fab抗體改建為SCFV后具有較好的特異性和親和力,但可溶性表達(dá)的ScFv的親和力低于可溶性Fab片段;The weight of ScFv about 750bp after VH linked with VL by SOE PCR. 經(jīng)重疊延伸PCR擴(kuò)增,將vH和vL連接后構(gòu)建的ScFv基因大約75obp;Results A recombinant phage ScFv library with liter of 4X109 pfu/ml was established. 結(jié)果 用M13KO7援救XL-Blue轉(zhuǎn)化菌中的pCANTAB5E重組噬菌體,得到滴度為4×10~9 pfu/ml噬菌體單鏈抗體庫;The overall yield of anti-AIF scFv with bioactivity in E. coli flask culture was more than 62 mg/L. 表達(dá)菌體的功能性AIF4c9scFv抗體產(chǎn)量約為62mg/L。Anti HBsAg ScFv genes have been successfully constructed and expressed in COS 7 cells. 成功地構(gòu)建了抗人HBsAg單鏈抗體基因 ,并可在COS 7細(xì)胞中分泌性表達(dá)Aim To increase the specificity and affi nity of a humanized mouse ScFv specific against human cross -linked fibrin. 目的提高人源化鼠抗人纖維蛋白單鏈抗體的親和力和特異性。 返回 scfv