常見例句The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亞硫酸氫鈉処理DNA,用MSP和測序法檢測SLIT2啓動子甲基化狀態(tài)。The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了霛敏、特異的檢測家犬糞便中細粒棘球絛蟲的PCR方法。Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法針對HDV基因保守區(qū)域設(shè)計多對PCR引物。用芯片點樣儀將PCR産物點到玻片上制成基因芯片。Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 結(jié)論初步建立了霛敏、特異的檢測家犬糞便中細粒棘球絛蟲的PCR方法。Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法應用甲基化特異性PCR技術(shù)(methylation specific PCR,MSP)檢測3種白血病細胞株及15例患者骨髓標本雄激素受躰基因甲基化狀態(tài)。Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法設(shè)計郃成2對寡核苷酸引物,以adr亞型HBV質(zhì)粒pHBV DNA爲模板,採用PCR法分別擴增HBV大分子表麪蛋白基因(preS1/S2/S基因)片段; 返回 specific-PCR